Department Combustion- and Particle Technology

• Head of Working group Air Liquid Exposure Systems

Key activities:

• Development of the Karlsruhe Exposure System for the deposition of nanoparticles on cell cultures
• Measurement of the particle dose on cell culture surfaces
• Development of dosimetry methods for the determination of particle doses on cell cultures
• Application of the Karlsruhe Exposure System to assess the biological responses caused by particles from combustion processes

CV:

Recent publications:

• Mülhopt S, Schlager C, Berger M, Murugadoss S, Hoet P. H., Krebs T, et. al. (2020)
  A novel TEM grid sampler for airborne particles to measure the cell culture surface dose.
  Scientific reports 10 (1), S. 8401. DOI: 10.1038/s41598-020-65427-w.
   
• Mülhopt S, Diabaté S, Dilger M, Adelhelm C, Anderlohr C, Bergfeldt T, et. al. (2018)
  Characterization of nanoparticle Batch-to-Batch Variability.
  Nanomaterials (Basel, Switzerland) 8 (5). DOI: 10.3390/nano8050311.

• Kanashova T, Sippula O, Oeder S, Streibel T, Passig J, Czech H, Kaoma T, Sapcariu SC, Dilger M, Paur H-R, Schlager C, Mülhopt S, Weiss C, Schmidt-Weber C, Traidl-Hoffmann C, Michalke B, Krebs T, Karg E, Jakobi G, Scholtes S, Schnelle-Kreis J, Sklorz M, Orasche J, Müller L, Reda A, Rüger C, Neumann A, Abbaszade G, Radischat C, Hiller K, Grigonyte J, Kortelainen M, Kuuspalo K, Lamberg H, Leskinen J, Nuutinen I, Torvela T, Tissari J, Javala P, Kasurinen S, Uski O, Hirvonen M-R, Buters J, Dittmar G, Jokiniemi J, Zimmermann R. (2018).
  Emissions from a Modern Log wood Masonry Heater and Wood Pellet Boiler: Composition and Biological Impact on Air-Liquid Interface Exposed Human Lung Cancer Cells.
  Journal of Molecular and Clinical Medicine 1(1):23–35.
   
• Langevin D, Lozano O, Salvati A, Kestens V, Monopoli M, Raspaud E, Mariot S, Salonen A, Thomas S, Driessen M, Haase A, Nelissen I, Smisdom N, Pompa PP, Maiorano G, Puntes V, Puchowicz D, Stępnik M, Suárez G, Riediker M, Benetti F, Mičetić I, Venturini M, Kreyling WG, van der Zande M, Bouwmeester H, Milani S, Rädler JO, Mülhopt S, Lynch I, Dawson K. (2018). Inter-laboratory comparison of nanoparticle size measurements using dynamic light scattering and differential centrifugal sedimentation. Nanoimpact 10. S. 97–107. DOI:10.1016/j.impact.2017.12.004.
   
• Krebs T, Mülhopt S, Diabaté S, Schlager C, Paur H-R, Dilger M. (2017). In Vitro Exposure Systems to Assess the Toxicity of Airborne Substances. In: Rihn BH, editor. BIOMEDICAL APPLICATION OF NANOPARTICLES. [S.l.]: CRC PRESS.
   
• Sapcariu SC, Kanashova T, Dilger M, Diabate S, Oeder S, Passig J, Radischat C, Buters J, Sippula O, Streibel T, Paur HR, Schlager C, Mülhopt S, Stengel B, Rabe R, Harndorf H, Krebs T, Karg E, Groger T, Weiss C, Dittmar G, Hiller K, Zimmermann R. (2016). Metabolic Profiling as Well as Stable Isotope Assisted Metabolic and Proteomic Analysis of RAW 264.7 Macrophages Exposed to Ship Engine Aerosol Emissions: Different Effects of Heavy Fuel Oil and Refined Diesel Fuel. PLOS ONE 11(6):e0157964.
   
• Mülhopt S, Paur H-R, Schlager C. 17.12.2014. Vorrichtung zur Messung von Feinstpartikelmassen (DE 10 2014 118 846 B4).
   
• Mülhopt, S., Dilger, M., Diabaté, S., Schlager, C., Krebs, T., Zimmermann, R., Buters, J., Oeder, S., Wäscher, T., Weiss, C., & Paur, H.-R. (2016). Toxicity testing of combustion aerosols at the air-liquid interface with a self-contained and easy-to-use exposure system. Journal of Aerosol Science, 96, 18. doi: http://dx.doi.org/10.1016/j.jaerosci.2016.02.005
   
• Mülhopt, S., Diabate, S., Schlager, C., Dilger, M., Krebs, T., Weiss, C., & Paur, H.-R. (2015). Automatisierte und definierte Deposition von Aerosolen auf Zellkulturen für eine Bewertung der biologischen Wirksamkeit von Feinstäuben. In V. W. GmbH (Ed.), Neue Entwicklungen bei der Messung und Beurteilung der Luftqualität (Vol. 2250, pp. 243-247). Düsseldorf: VDI Verlag GmbH.
   
• Mülhopt, S., Krebs, T., Diabaté, S., Schlager, C., & Paur, H.-R. (2015). Feinstaub in der Lunge - Automatisiertes Expositionssystem für Zellkulturen an der Gas-Flüssigkeits-Grenzschicht. labor&more, 02/15, 32-37. http://www.laborundmore.com/research/6468,804272/Sonja-Muelhopt/Automatisiertes-Expositionssystem-fuer-Zellkulturen-an-der-Gas-Fluessigkeits-Grenzschicht.html  
   
• Oeder, S., Kanashova, T., Sippula, O., Sapcariu, S. C., Streibel, T., Arteaga-Salas, J. M., Passig, J., Dilger, M., Paur, H.-R., Schlager, C., Mülhopt, S., Diabaté, S., Weiss, C., Stengel, B., Rabe, R., Harndorf, H., Torvela, T., Jokiniemi, J. K., Hirvonen, M.-R., Schmidt-Weber, C., Traidl-Hoffmann, C., BéruBé, K. A., Wlodarczyk, A. J., Prytherch, Z., Michalke, B., Krebs, T., Prévôt, A. S. H., Kelbg, M., Tiggesbäumker, J., Karg, E., Jakobi, G., Scholtes, S., Schnelle-Kreis, J., Lintelmann, J., Matuschek, G., Sklorz, M., Klingbeil, S., Orasche, J., Richthammer, P., Müller, L., Elsasser, M., Reda, A., Gröger, T., Weggler, B., Schwemer, T., Czech, H., Rüger, C. P., Abbaszade, G., Radischat, C., Hiller, K., Buters, J. T. M., Dittmar, G., & Zimmermann, R. (2015). Particulate Matter from Both Heavy Fuel Oil and Diesel Fuel Shipping Emissions Show Strong Biological Effects on Human Lung Cells at Realistic and Comparable In Vitro Exposure Conditions. PLOS ONE, 10(6), e0126536. doi: 10.1371/journal.pone.0126536
   
• Panas, A., Comouth, A., Saathoff, H., Leisner, T., Al-Rawi, M., Simon, M., Seemann, G., Dössel, O., Mülhopt, S., Paur, H.-R., Fritsch-Decker, S., Weiss, C., & Diabaté, S. (2014). Silica nanoparticles are less toxic to human lung cells when deposited at the air–liquid interface compared to conventional submerged exposure. Beilstein Journal of Nanotechnology, 5, 1590-1602. doi: 10.3762/bjnano.5.171
   
• Comouth, A., Saathoff, H., Naumann, K.-H., Mülhopt, S., Paur, H.-R., & Leisner, T. (2013). Modelling and measurement of particle deposition for cell exposure at the air liquid interface. Journal of Aerosol Science, 63, 103-114. doi: 10.1016/j.jaerosci.2013.04.009
   
• Panas, A., Marquardt, C., Nalcaci, O., Bockhorn, H., Baumann, W., Paur, H.-R., Mülhopt, S., Diabaté, S., & Weiss, C. (2013). Screening of different metal oxide nanoparticles reveals selective toxicity and inflammatory potential of silica nanoparticles in lung epithelial cells and macrophages. Nanotoxicology, 7(3), 259-273. doi: 10.3109/17435390.2011.652206